NOTE:

If you are interested in trying out the PetakaG3 in your lab then please contact our Customer Service Department to request free samples.

Key Features:

• No additional CO₂ required for any type of media although the PetakaG3 can also be used in CO₂ incubators
• No added humidity required (not influenced by environmental dehydration)
• Compact (127.5 mm x 85.5 mm x 5 mm)
• The most secure cell culture device against contamination available today (no caps, no openings, no spills)
• Robust structure (without breakable films)
• Excellent for shipping live cells without requiring freezing and dry ice
• Optimizes media and growth factor supplies
• Produces 8 times more media volume per incubator capacity
• Provides 15 times more cell culture surface per incubator capacity
• In vitro cell dormancy is easily accomplished
• Allows storage of pre-filled units that are ready to host cells
• Operates in any position

The PetakaG3 is a cell culture device designed to culture adherent and non-adherent cells. The device eliminates all air space inside the culture chamber to allow the anchorage and growth of adherent cells on all internal surfaces.

The PetakaG3 is especially designed to grow cells in an isolated, auto-modified milieu, that is highly protected from external environmental conditions. That protection permits effective cell incubation with or without external  CO₂ control and independent of relative humidity in the atmosphere. A proprietary structural design establishes a gas exchange rate that provides a suitable growing environment for the majority of cells incubated in regular atmosphere (0.2% CO₂). Additionally, this structure reduces dehydration to virtually zero which allows the storage of media preloaded devices for weeks. Therefore the PetakaG3 can be effectively incubated in any temperature-controlled chamber without CO₂ and humidity controlled environment.

The internal cell culture chamber has 150 cm² of surface area available for cell attachment and growth and 20 ml (±1%) of volume. This optimized media/surface area rate (0.133 ml/cm²) facilitates the growth of large numbers of cells per PetakaG3 (the number is dependent upon the cellular metabolic and doubling time characteristics of the cells) without media replacement.  This allows for optimal use of media and special additives such as growth factors.

PetakaG3 Versions

There are five versions of the PetakaG3:

- PetakaG3™ LOT (Low Oxygen Transfer
- PetakaG3™ ET™ (Easy Transfer)
- PetakaG3™ HOT (High Oxygen Transfer)
- PetakaG3™ FLAT Suspended Cell Culture)
- PetakaG3™ MATRIX  (Specific Molecular Matrix)

The PetakaG3 HOT, FLAT and MATRIX are manufactured from a co-polymer called Styrolux and are specifically designed for high oxygen respiring cells such as liver cells, monoclonal hybridomas, etc. Cells can be put into dormancy for transportation or short-term storage but the high gas diffusion material of the PetakG3 HOT does not allow the oxygen level to go below 2%; it is therefore unsuitable for long-term dormancy (more than 30 days without media change). Culturing for more than two weeks is also not recommended as the PetakaG3 walls not only allow gas to diffuse through but they also allow minute amounts of water to escape.

The PetakaG3™ FLAT bioreactor has all of the same size and structural characteristics of the PetakaG3™ HOT bioreactor except that the interior surfaces are not treated to promote cell adherence to the walls. The PetakaG3™ MATRIX is also similarly constructed to HOT but in this version the internal surfaces are coated with a specific molecular matrix such as collagen, fibronectin, poly-L-lysine, Matrigel, gelatin and laminin. This product is best suited to growing different types of stem cells.

The PetakaG3 LOT and ET are manufactured from a crystal polystyrene called Styron and are designed for most mammalian cells with a low to medium oxygen diffusion rate such as CHO cells. This is the material from which all previous versions of the Petaka were manufactured. This material allows up to two months of dormancy without media change of low/medium oxygen respiring cells and up to several months with media change. Culturing in PetakaG3 LOT brings the dissolved oxygen levels in the media down to 1.5% that induces dormancy at confluence.

The PetakaG3™ ET™ bioreactor has all of the same basic characteristics as the PetakaG3™ LOT but contains magnetic particles inside the main cell culture chamber.The Petaka™ ET bioreactor is used in conjunction with the ClearCell™ instrument to mechanically detach cells with minimal cell damage. This allows cell harvesting without using enzymes or chemicals such as Trypsin-EDTA and the ET version is well suited for culturing cells with special protocols such as those for stem cell cultures.

System Accessories

• lluminated LCD screen
• Centrifugation speed up to 20,000 rpm
• Allows the regulation of rpm in increments of 100 rpm,  in increments of 10 x g, & time setting of 1 to 99 minutes or indefinite time setting
• Programmable brake regulator from between 5 to 180 seconds, in 5 second increments.
• Programmable with 16 simultaneous programs
• Countdown from attaining selected RPM/RCF or from zero.
• Graphical & numerical visual displays
• Push buttons of opening of the lid, start, stop, enter and exit.
• Constant display of the status of the apparatus
• Programmable opening: automatically at the end of the cycle or manually by pressing the push button on the panel.
• Adjustable acceleration: smooth & fast

• Luer-lock plastic hub
• 12.7 mm stainless steel tip
• 18 Gauge with blunt end for safety
• OD = 1.26 mm and ID = 0.7 mm
• Sterile and autoclavable

Videos

• Culturing adherent cells
• Detaching cells from the Petaka for splitting without using a centrifuge
• Filling the Petaka
• Hypoxic studies using the Petaka
• Introducing Trypsin to the Petaka
• Cell seeding with the Petaka
• Keeping cells in in-vitro dormancy: Putting cells in dormancy

• Coating Petakas with Gelatin
• Harvesting and seeding cells using the PetakaG3 ET
• Hybridoma cell culture with the Petaka HOT
• Magneto-assisted cell transfection
• Magnetofection with Polymag Neo
• Transfection using DreamFect Gold and the PetakaG3
• Umbilical cord blood processing

Key Features:

• Easy to install, program and operate
• Inexpensive to use
• Employs single-use, disposable CellexusBag cell culture bags that eliminate cross-contamination and minimize system down-time
• No cleaning or autoclaving required
• Highly accurate temperature control (15°C - 40°C) with built-in chiller to enable heating and cooling of culture media with no external equipment needed
• Oxygen generation capability (up to 4 litres/minute) eliminates the need and extra cost of an external gas supply
• Efficient airlift technology delivers effective, controllable aeration and mixing with minimal shearing. This eliminates cell settling and enhances protein yields
• Programmable air/enriched air flow rate and high efficiency of oxygen transfer (1-10 litres per minute) to improve protein yields
• Flexible culture/sample ports for easy inoculation and removal of culture samples
• Single-use CellexusBag manufactured under ISO 10000 conditions to provide quality assurance

CellexusBag single use bioreactor bags

The CellMaker PLUS is a state-of-the-art, easy to use, single-use, disposable bioreactor control system. The unit is fully automated in order to speed up and simplify cell culture and fermentation bioprocesses and includes a wealth of new technologies for efficient growth of the widest range of cell types. It is an innovative, high quality, robust system which can be used with bacteria, yeast, algae, bacteriophage as well as the more challenging mammalian and insect cells. Cells are cultured in a unique, single-use, disposable cell culture bag, the CellexusBag™, that expedites set-up times and eliminates the extensive cleaning and sterilizing requirements of other systems.



CellMaker Plus condenser system and optional condenser liner

Unlike other disposable systems, which need shakers, rockers, rollers or large environmental chambers, the CellMaker PLUS eliminates mechanical methods for mixing by employing a proprietary airlift technology for optimal mixing and oxygenation of the cell culture. This is highly efficient, quick, simple, scalable and inexpensive to use. It provides optimal aeration along with precise temperature control enabling production of high cell densities and maximizing expression levels of soluble protein. The CellMaker Plus consists of four modules: an enclosure, a controller, a supply hub and an oxygenator:

Enclosure

The Enclosure holds the single use CellexusBag and incorporates a Peltier driven thermal plate heater, a built in chiller and a PT100 temperature sensor to enable cell culture temperature to be closely monitored and controlled. Enclosures are available in 8 L and 50 L sizes for use with 1-8 L and 10-50 L CellexusBags in order to provide scale flexibility. A high efficiency condenser system removes moisture from an exhaust line before it is vented through a microbiological air filter and optional gamma-irradiated, single-use condenser liners are available to provide ease of use and reduced set-up/turn-around times. The Enclosure also incorporates transparent panels to allow visualization of culture progress and to enhance performance for light-dependent applications.

CellMaker Enclosure

Controller

Key protocol parameters are entered into the controller via a 15" touchscreen monitor. Control of dissolved oxygen (DO) is essential to achieve optimal cell concentrations and to ensure the viability of mammalian and insect cells and so the Controller is available with an optional pH and oxygen sensor reader (which can be retrofitted) in order to measure pH and dissolved oxygen concentration. Automated feedback of data enables the Controller to adjust the pH of the culture and to regulate air, oxygen and carbon dioxide flow.

SupplyHub

The SupplyHub incorporates an air pumping system, temperature regulation as well as process measurement and data acquisition. It also provides a power source to the Controller and Enclosure.

Oxygenator

The Provides a continuous supply of oxygen and can dovetail with the SupplyHub or be used as a stand-alone unit.

More Information

Please click here to view a video of the CellMaker Plus or here to download a brochure. Contact Discovery for pricing.