Real-time qPCR system, real-time pcr, real-time fluorescence detection, Quantitative PCR, qPCR detection, FRET filters, qTOWER, Analytik Jena

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qTOWER 2.0 and 2.2 advanced real-time qPCR systems

Key Features:

Available in standard (2.0) or gradient versions (2.2)
Optimized for volumes of 10 – 60 µl
Can be configured individually with up to 6 different measurement channels
Choice of 10 high-resolution qPCR excitation and emission filters (colour or FRET modules). Filters can be added to the unit after purchase as required.
High-speed scanning rate of 6 seconds for a 96 well microplate independent of the number of dyes to be measured
Heating rates up to 5.5^oC/sec and cooling rates up to 4^oC/sec
Sensitivity of 1nM FAM in minimal 30 uL sample volume
Intuitive qPCRsoft software provides instrument control, protocol development and data analysis capability

The qTOWER 2.0 and 2.2 have been designed to allow the use of standard PCR consumables such as 0.2 mL single tubes, 8-well strips of 0.2 mL tubes or 96-well PCR microplates. The qTOWER 2.0 and 2.2 work with 3 independent, long-life, stable blue, white and red LEDs to optimally excite fluorescence dyes over a wide spectral range. This ensures that the highest possible quantum yield is achieved in each real-time PCR experiment. The qTOWER 2.0 and 2.2 can easily process sophisticated multiplex experiments with up to 6 different fluorescence-tagged probes ranging from blue to the far-red spectral range. The patented optical system used for this application consists of a shuttle with 8 high performance fibers which guarantee that the 96 well block can be read out quickly in only 6 seconds independent of the number of dyes to be measured.

Ten different colour and FRET modules are available:

Name	Excitation	Emission	Dyes

Module 1	470 nm	520 nm	FAM, Sybr Green, Alexa 488
Module 2	515 nm	545 nm	JOE, HEX, VIC, Yakima Yellow
Module 3	535 nm	580 nm	TAMRA, DFO, Alexa546, NED
Module 4	565 nm	605 nm	ROX, Texas Red, Cy 3.5
Module 5	630 nm	670 nm	Cy5, Alexa 633, Quasar 670
Module 6	660 nm	705 nm	Cy5.5, LightCylcer Red
FRET 1	470 nm	580 nm	FAM (donor) / TAMRA (acceptor)
FRET 2	470 nm	670 nm	FAM (donor) / Cy5 (acceptor)
FRET 3	470 nm	705 nm	FAM (donor) / Cy5.5 (acceptor)
FRET 4	515 nm	670 nm	JOE (donor) / Cy5 (acceptor)

The qTOWER 2.0 and 2.2 have a 96 well, gold-anodized, silver block. The block temperature range is 3^oC – 99^oC. Temperature control modes include block control or tube control (simulated). The lid is heated up to 110^oC.

The qTOWER 2.0 is supplied with qPCRsoft software for instrument control, protocol development and data analysis. qPCRsoft includes numerous methods for evaluating fluorescence data. This includes determination of PCR efficiencies and performance of absolute and relative quantifications. Other analytical techniques include delta-delta Ct method that allows for the determination of the relative expression level of a gene of interest in relation to one or more reference genes. End-users can also use qPCRsoft to investigate reliable concentrations and precise allele conditions and to display exact expression ratios. Once defined, parameter sets can be applied as templates for future applications and be reused continuously. There is no limit to the number of protocols that can be stored on the laptop. Upgrades to the qPCRsoft are provided at no charge to the end-user.

More Information

A brochure describing the qTOWER 2.0 and 2.2 is available for download. Please contact Discovery for pricing.

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	qTOWER advanced real-time qPCR system Key Features Real-time PCR and analysis of 96 samples in parallel within less than 60 minutes Sensitivity down to 1 nM FAM in minimal 15 uL PCR buffer (equals 15 fmol FAM per well) Multi-component analysis Heating rates of up to 12°C/sec and cooling rates of up to 8°C/sec Work with samples as small as 5 µL 9 different colour modules available including 4 FRET filters. Equipped with up to four colours. Read-out of 96 wells within 4 sec regardless of the number of dyes Integrated real-time software features automatic data analysis with different methods for quantification, expression ratios and PCR efficiency Absolute and relative quantification The qTOWER advanced real-time qPCR system has been developed by Analytik Jena of Jena, Germany, and is based on a novel fiber optic system patented by the company. The qTOWER uses high power long-life LED light sources and a highly sensitive channel photomultiplier. The unit combines real-time fluorescence detection with Analytik Jena's super-fast rapidPCR technology that can generate ramping rates of up to 12 °C/sec at the same time as considerably reducing sample volume down to as low as 5 µL per reaction. The qTOWER is a highly flexible instrument offering nine different colour modules that make it ideal for multiplexed applications using commercially available fluorescent dyes. More Information A video of the qTOWER can be view by clicking here. Please click here to download a PDF copy of the qTOWER brochure or here for a PDF of the Technical Data brochure for this product.

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